Heterokaryosis and you may parasexual recombination inside pathogenic stresses of Fusarium oxysponrm
V. Heterokaryosis and you can parasexuality
Use the “0”spot for one of the two parents and notice the stress amount on dish. Make use of the layout with the replicator. Incubate 2-three days. Simulate the fresh segregants for the a number of decide to try plates using a replicator which have, age.grams., 21 needles. Draw the new dishes having a variety. Incubate 2-three days. Score the exam dishes and record the newest phenotypes regarding the rating dining table. You will need to influence this new ploidy of the territories to your base away from the latest indicators. Read the ploidy away from unsure colonies. Generate a list of this new genotypes (you can make use of a computer program). Dictate the fresh portion of the new recombinants to the some other markers. Which indicators try linked? Are you willing to look for intrachromosomal recombination? In which linkage class ‘s the not familiar marker?
Inside test we determine the fresh gene purchase and you will place of the new centromere when you look at the linkage class VI ofA. niger.Individuals methods for your choice of mitotic recombinants are utilized. New indicators inside try: pubA1, pyrB4, c d l . New c d locus try terminal towards chromosome case and for this reason very suitable because the alternatives marker. Given that every indicators try recessive, they must be in the cis position. New chlorate-unwilling segregants would be remote, and end up being examined to the almost every other markers. The fresh diploid made use of is: N761 N640